{"id":19,"date":"2018-11-28T03:20:19","date_gmt":"2018-11-28T03:20:19","guid":{"rendered":"http:\/\/noralab.ucsf.edu\/?page_id=19"},"modified":"2021-08-31T17:13:32","modified_gmt":"2021-08-31T17:13:32","slug":"protocols","status":"publish","type":"page","link":"https:\/\/noralab.ucsf.edu\/index.php\/protocols\/","title":{"rendered":"Resources"},"content":{"rendered":"\n

Protocols<\/h1>\n\n\n\n
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Protocols are constantly evolving.
Feel free to reach out for the most up to date version or if you have suggestion to improve them.<\/p>\n\n\n

Tissue Culture and Genome Editing<\/h3>\n\n\n

Culture of feeder-free mouse embryonic stem cells<\/a><\/p><\/blockquote>\n\n\n\n

Transfecting mouse embryonic stem cells for genome editing<\/a><\/p><\/blockquote>\n\n\n\n

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Reagents<\/h1>\n\n\n\n

Plasmids<\/h3>\n\n\n\n

Feel free to reach out, we have lots of unpublished variations of these vectors<\/em><\/h6>\n\n\n\n

Addgene link of the lab<\/strong>:<\/a>
– various doxycycline-inducible mouse CTCF cDNAs mutants<\/strong>
– lots of mouse cohesin cDNAs<\/strong> (published in Nora, Caccianini, Fudenberg et al. 2020)
– gene targeting of various cohesin-related proteins (including mouse RAD21<\/strong> and SORORIN<\/strong>, for tagging with Halotag<\/strong>, auxin-inducible degron<\/strong>, …)
– and more!<\/p><\/blockquote>\n\n\n\n

Addgene link of the Bruneau lab<\/a> <\/strong>
– plasmids to implement the auxin-inducible degron system<\/strong> in mammalian cells and reagents to target mouse CTCF <\/strong>(published in Nora et al. 2017)<\/p><\/blockquote>\n\n\n\n

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Cell lines<\/h3>\n\n\n\n

Feel free to reach out, we have lots of unpublished variations of these cell lines<\/em><\/h6>\n\n\n\n

Genome-edited mouse embryonic stem cell lines published by the lab<\/a> <\/strong><\/p><\/blockquote>\n\n\n\n

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Some Science Resources We Like<\/h1>\n\n\n\n

e-learning<\/h2>\n\n\n\n